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A five-month study in mice administering 20% D-mannose via drinking water reported no adverse effects on body weight, organ function, metabolism, or reproductive parameters, suggesting good tolerance even at high doses 26

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[13] Most mammalian isoenzymes have affinity for the substrate 1-chloro-2,4-dinitrobenzene, and spectrophotometric assays utilising this substrate are commonly used to report GST activity